Trans-stilbenoxide, enantiomeric separation
CHIRALYSER
RANGE: 8 mdeg
AVERAGE: 0.2 sec
Sample: Trans-stilbenoxide
Column: Chira-GROM-2, 15µm, 250×4.6
Eluent: Heptane/2-Propanol (95/5), 1ml/min
Inject: 5 µl (0.5g/l)
Chlormezanone, enantiometric separation
CHIRALYSER
RANGE: 8 mdeg
AVERAGE: 0.2 sec
Sample: Chlormezanone
Column: Chira-GROM-3, 15µm, 250×4.6
Eluent: EtOH/ACN (99/1), 0.6 ml/min
Inject: 3 µl (1.6g/l)
Oxazepam, enatiomeric separation
CHIRALYSER
RANGE: 8 mdeg
AVERAGE: 0.2 sec
Sample: Oxazepam
Column: Chira-GROM-2, 15µm, 250×4.6
Eluent: ACN/H2O/TFA (50/49.9/0.1), 0.6ml/min
Inject: 10 µl (1.2g/l)
5-Methly-5-phenylhydantoin versus UV
CHIRALYSER
RANGE: 4 mdeg
AVERAGE: 0.5 sec
Sample: 5-Methyl-5-phenylhydantoin
Column: Chirobiotic T, 250×4.6
Eluent: MeOH, 1ml/min
Inject: 10 µl (1g/l)
Ketoprofene, various concentration
CHIRALYSER
RANGE: various
AVERAGE: 0.1 sec
Sample: Ketoprofene
Column: Chirapak AD, 250×4.6
Eluent: n-hexane/EtOH/TFA (80/20/0.01), 1ml/min
Inject: 20µl, various concentration
Androgene, mixture
CHIRALYSER
RANGE: 8 mdeg
AVERAGE: 0.1 sec
Sample: Androgene (mixture)
Column: LiChrosphere, 125×4.6
Eluent: ACN/H2O (70/30), 1ml/min
Inject: 5 µg/each in 20 µl
Enatiomeric excess, various e:e
POLARmonitor
RANGE: 0128
AVERAGE: 10
Sample:
(+)-1-Phenyl-1-propanol
(-)-1-Phenyl-1-propanol
Column: LiChrosphere, 250×4.6
Eluent: MeOH/H2O (80/20), 1ml/min
Inject: 10 µl (1g/l) various e:e
Optical purity, UV versus rotation
POLARmonitor
RANGE: 0128
AVERAGE: 05
Sample: see structure
Column: Chira-GROM-2, 15µm, 250×4.6
Eluent: Heptane/2-Propanol (95/5), 1ml/min
Inject: 0.44 mg in 20 ml
Carbohydrates, mixture
POLARmonitor
RANGE: 0064
AVERAGE: 10
Sample: Carbohydrates (mixture)
Column: Hypersil APS, 125×4.6
Eluent: ACN/H2O (80/20), 0.5ml/min
Inject: 20 ml (1g/l)
Labetalol, 2 diastereometric pairs
CHIRALYSER
RANGE: 4 mdeg
AVERAGE: 0.2 sec
Sample: Labetalol
Column: Chirobiotic V, 250×4.6
Eluent: MeOH/AFTA (100/0.1), 1ml/min
Inject: 10µl (1g/l)